Plasmid

Part:BBa_K5048023

Designed by: Yecheng Zhao   Group: iGEM24_WHU-China   (2024-09-14)


plasmid AQ_GST

This plasmid uses pET-28a as backbone. This part is the surface-decorating system which display small peptide AQ in the outer leaflet of the outer-membrane of E.coli. The DDDDK enterokinase cleavage site can be recognized by enteroskinase and small peptides will be cut off. It contains the T7 promoter, Lac operator, lpp'OmpA(codon optimised), enterokinase site, AQ, GST tag, rrnB T1 terminator and T7Te terminator.It is used in the verification of the enzymatic cleavage system

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6301
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6301
    Illegal NotI site found at 6326
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6301
    Illegal BglII site found at 4964
    Illegal BamHI site found at 6295
    Illegal XhoI site found at 6335
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6301
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6301
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 3184
    Illegal NgoMIV site found at 3344
    Illegal NgoMIV site found at 4932
    Illegal AgeI site found at 5409
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2263


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Categories
Parameters
None